Thermo Fisher Scientific DDDDK Polyclonal Antibody, Biotin
DDDDK(FLAG) 태그 단백질 검출용 비오틴 결합 폴리클로날 항체. Western blot, ELISA, IP 등에 적합하며, 토끼 유래 IgG 형식. 합성 DYKDDDDK 펩타이드 면역원으로 제작되어 높은 특이성과 재현성 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to DDDDK tag (Sequence: DYKDDDDK) |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence used as a protein tag to facilitate purification, detection, and localization.
The tag consists of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) and is typically fused to the N- or C-terminus of a protein of interest.
This allows easy identification and isolation using anti-FLAG antibodies. The FLAG tag is widely used in molecular biology and biochemistry research for applications such as protein-protein interaction studies, expression analysis, and localization studies.
The FLAG tag is designed to be hydrophilic, minimizing interference with protein folding or activity.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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