Thermo Fisher Scientific PMCA1 ATPase Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.03-0.1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide with sequence C-KQDGAIENRNKAKAQD. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PMCA1 ATPase,uniProtId:P20020-3,ncbiNodeId:9606,antigenRange:312-327,antigenLength:1220,antigenImageFileName:PA5-142821_PMCA1_ATPase_P20020-3_Goat.svg,antigenImageFileNamePDP:PA5-142821_PMCA1_ATPase_P20020-3_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2933464

Product Specific Information

Additional Information: This antibody is expected to recognize both reported isoforms (NP_001001323.1; NP_001673.2).

This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:64,000.

Target Information

The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/potassium pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.