Thermo Fisher Scientific Human NEK1, GST Tag Recombinant Protein

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

1-505

Tag

GST-tag

Molecular weight

85.3 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris, pH 7.5, with 50% glycerol, 0.02% Triton X-100, 150mM NaCl, 0.5mM EDTA, 2mM DTT

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

Protein Form: Catalytic

Target Information

NEK1 is a serine/threonine protein kinase, related to the NIMA cell cycle regulator, and is highly expressed in meiotic germ cells. The kinase may function early in the DNA damage response pathway. NEK1 may also participate in polycystic kidney disease and neural cell development. Like most members, the encoded protein contains an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.