Thermo Fisher Scientific BrdU Monoclonal Antibody (OTI7H5), TrueMAB
BrdU 검출용 Rat 모노클로날 항체로 세포 증식 분석에 적합. 파라핀 포매 조직 IHC에 사용 가능. Lyophilized 형태로 제공되며 PBS 버퍼 포함. 연구용으로만 사용.
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Thermo Fisher Scientific BrdU Monoclonal Antibody (OTI7H5), TrueMAB
Applications
- Immunohistochemistry (Paraffin) (IHC (P))
Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Rat / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7H5 |
| Immunogen | Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside and thymidine analogue.
BrdU is used to detect proliferating cells in living tissues by incorporating into newly synthesized DNA during the S phase of the cell cycle.
BrdU-specific antibodies detect incorporated BrdU, identifying actively replicating cells.
Binding of the antibody requires DNA denaturation, typically achieved by acid or heat treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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