Thermo Fisher Scientific BrdU Monoclonal Antibody (OTI7H5), TrueMAB

Thermo Fisher Scientific BrdU Monoclonal Antibody (OTI7H5), TrueMAB

Applications

• Immunohistochemistry (Paraffin) (IHC (P))

Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside and thymidine analogue.
BrdU is used to detect proliferating cells in living tissues by incorporating into newly synthesized DNA during the S phase of the cell cycle.
BrdU-specific antibodies detect incorporated BrdU, identifying actively replicating cells.
Binding of the antibody requires DNA denaturation, typically achieved by acid or heat treatment.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.