Thermo Fisher Scientific Smoothelin Polyclonal Antibody
Rabbit polyclonal antibody targeting human Smoothelin for WB and IHC applications. Recognizes fully differentiated smooth muscle cells. High specificity and affinity-purified. Supplied as liquid form with 0.2 mg/mL concentration, stored at 4°C or -20°C.
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Applications
Western Blot (WB)
- Tested Dilution: 0.04–0.4 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:5,000–1:10,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human Smoothelin. Recombinant protein control fragment (Product #RP-103107) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2647656 |
Product Specific Information
Immunogen sequence:
LAGLDEGALR KLLEVTADLA ERRRIRSAIR ELQRQELERE EEALASKRFR AERQDNKENW LHSQQREAEQ RAALARLAGQ LESMNDVEEL TALLR
Antigen sequence identity:
- Mouse: 97%
- Rat: 96%
Target Information
Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses reveal a broad species distribution of this protein.
Cells with SMC-like characteristics, such as myofibroblasts, myoepithelial cells, and skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy and transfection studies show a filamentous organization distinct from desmin and vimentin. Smoothelin colocalizes with actin stress fibers.
Two tissue-specific isoforms have been identified:
- 59 kDa isoform specific for visceral SMC
- 100 kDa isoform specific for vascular SMC
Human smoothelin is encoded by a single copy gene located on chromosome 22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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