Thermo Fisher Scientific ATP6V1F Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:500–1:1,000

Product Specifications

Product Specific Information

• Immunogen sequence: DTFRSLGSLP GSVVEANPNQ RDPPLWDEID SRQFL
• Highest antigen sequence identity to orthologs:
  • Mouse: 34%
  • Rat: 37%

Target Information

ATP6V1F encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase–dependent organelle acidification is essential for intracellular processes such as:

• Protein sorting
• Zymogen activation
• Receptor-mediated endocytosis
• Synaptic vesicle proton gradient generation

V-ATPase is composed of:

• V1 domain (cytosolic): Three A and three B subunits, two G subunits, plus C, D, E, F, and H subunits — contains ATP catalytic site
• V0 domain (transmembrane): Five different subunits (a, c, c′, c″, and d)

Additional isoforms of V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. The encoded protein is the V1 domain F subunit protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.