Invitrogen™

Platinum™ Tfi Exo(-) DNA Polymerase

Platinum™ Tfi Exo(-) DNA Polymerase is recombinant Tfi Exo(-) DNA Polymerase complexed with a proprietary antibody mix that inhibits polymerase자세히 알아보기

Platinum™ Tfi Exo(-) DNA Polymerase is recombinant Tfi Exo(-) DNA Polymerase complexed with a proprietary antibody mix that inhibits polymerase activity at ambient temperature, allowing room-temperature reaction setup. Activity is restored after the initial denaturation step in PCR cycling at 94°C, providing an automatic hot start for increased specificity, sensitivity, and yield. Tfi Exo(-) DNA Polymerase is purified from E. coli. expressing cloned mutants of the Thermus filiformis DNA polymerase gene. This enzyme has 5→3 polymerase activity, but lacks both 5→3 and 3→5 exonuclease activity for improved yield and robustness. Tfi Exo(-) DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from single-stranded templates in the presence of a primer.

Applications
Platinum™ Tfi Exo(-) DNA Polymerase can be used in protocols that currently use Platinum™ Taq DNA Polymerase without modification. PCR performance is comparable to that of Platinum™ Taq in yield, specificity, fidelity, and robustness. Like Platinum™ Taq, Platinum™ Tfi Exo(-) DNA Polymerase has a nontemplate-dependent terminal transferase activity that adds a single deoxyadenosine (A) to the 3` ends of PCR products.

사양

GC-Rich PCR PerformanceLow

중합효소Tfi DNA Polymerase

반응 속도Standard

제품라인Platinum™

제품 유형DNA Polymerase

수량500 units

배송 조건Dry Ice

농도5X

Fidelity (Taq 대비)1 X

핫 스타트Built-In Hot Start

오버행3`-A

반응 형식Separate Components

크기(최종 제품)1 kb or less

부피5.2 mL

Unit SizeEach