Thermo Fisher Scientific Goat anti-Human IgG Fc Recombinant Secondary Antibody, HRP
HRP 결합 염소 유래 항-인간 IgG Fc 재조합 2차 항체로 높은 특이성과 민감도를 제공. 인간 IgA, IgM 및 타종 면역글로불린과 교차반응 없음. ELISA, Western blot 등 다양한 면역분석에 적합. 로트 간 일관성과 동물 유래 성분 없음.
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- A55745x (2개 옵션)
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:2,000–1:10,000 |
| Peptide Array | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Secondary Antibody |
| Clone | 3H8L9 |
| Immunogen | Purified Human IgG |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with proprietary stabilizer |
| Contains | Proprietary preservative |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Wet ice |
| RRID | AB_2925770 |
| Target | IgG Fc |
| Antibody Form | Whole Antibody |
Additional Formats
- Alexa Fluor 488
- Alexa Fluor 647
- Alexa Fluor Plus 488
- Alexa Fluor Plus 647
- AP
- Biotin
- FITC
- PE
- TRITC
- Custom conjugation available upon request
Product Specific Information
This Goat anti-Human IgG Fc Recombinant Secondary Antibody, HRP specifically detects human IgG Fc with no cross-reactivity to human IgA, IgM, or immunoglobulins from other species.
Each lot's sensitivity and specificity are confirmed using ELISA.
Recombinant antibodies are produced using cloned genes encoding the desired antibody, expressed in vitro.
Advantages include:
- Enhanced specificity
- Animal origin-free formulation
- Lot-to-lot consistency
It is recommended to titrate the antibody for optimal performance in each assay.
Target Information
Anti-Human secondary antibodies are affinity-purified and specifically recognize human immunoglobulins.
They are useful for detection, sorting, or purification of human targets.
Secondary antibodies provide versatility by supporting multiple detection systems (e.g., HRP, AP, fluorescence) and offer improved sensitivity through signal amplification.
Typically, these antibodies are generated by immunizing host animals with pooled human immunoglobulins and may undergo further purification and modification (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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