
F-995 ORP FermProbe with K9 Metric Cap
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The F-995 Redox FermProbe features a K9 metric cap for use with red or orange cable connectors equipped with a K9 connector. Redox sensors are commonly used to maintain anaerobic conditions in culture media.
Reasons to Measure Redox in Fermentation Media
The metabolic activity of microorganisms depends on many factors, including the redox potential of the culture environment. Measuring the redox potential allows the vessel operator to monitor the addition of reducing agents while ensuring that the potential is within the proper range for initiation of growth. It is also important to monitor the redox potential just before inoculation.
Image Text
- pH Electrode
- Redox Electrode
- pH sensitive glass bulb
- Redox sensitive Pt band
Anaerobic Fermentation
Redox sensors are most commonly used to maintain anaerobic conditions in a culture medium. They can measure trace amounts of dissolved oxygen below 1 ppm, at levels that are too low for dissolved oxygen sensors.
Downstream Processing
Redox sensors can be used in downstream fermentation steps to monitor changes in concentration or the absence or presence of specific chemicals. Monitoring redox potential is an effective way to track chemical conversions during the process.
Protein Folding
Close regulation of redox potential is crucial for the efficient formation of disulfide bonds, which facilitate protein folding and stability. Overly oxidizing conditions can result in misfolding due to the formation of incorrect bonds.
Metabolic Pathways
Measuring redox potential is an effective way to determine its influence on the metabolic pathways of microorganisms. This is useful for substrate utilization or the production of specific metabolites.
Specification for F-995 Redox FermProbe
| Specification | Details |
|---|---|
| Measurement range | ±5000 mV |
| Sterilization | Steam sterilizable to 135°C |
| Maximum pressure | 150 psig |
| Reference system | Double junction, Ag-AgCl reference system |
| Disconnect cap | Metric Pg13.5 threaded disconnect cap |
| Connector compatibility | K9 connector; typically used with red or orange cable connectors |
| Cable requirement | Requires disconnect cable and connector assemblies |
How to Order a Redox Sensor
- Choose the sensor model:
If the sensor needs to fit an existing cable, check the cable connector and note its color. If the cable connector is black and has an S8 connector, use the F-915 or F-935 Redox FermProbe. If the cable connector is red or orange and has a K9 connector, use the F-995 Redox FermProbe. - Choose the sensor length:
If you have already chosen a housing, select a sensor length with the same class designation as the housing. The housing ordering information box also lists the correct sensor length to order. If you have not already chosen the housing, review the recommendations for each sensor model below. - Confirm cable length and connector.
Series F-900 Redox FermProbes
| Sensor model | Class | Compatible housing models | Sensor length | Sensor part number |
|---|---|---|---|---|
| F-915 | A | 320, 330, 326, 336, 350, 357, 370 | 130 mm | F-915-B130-DH |
| F-935 | D | 325, 335 | 120 mm | F-935-B120-DH |
| F-995 | D | 325, 335 | 120 mm | F-995-B120-DK |
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