Thermo Fisher Scientific CENPF Polyclonal Antibody, DyLight 650
CENPF 단백질을 인식하는 DyLight 650 결합 폴리클로날 항체로, Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합합니다. 인간, 마우스, 소, 말 시료에 반응하며, 651/673 nm 파장 특성을 가집니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:200–1:500 |
| Flow Cytometry (Flow) | 1 µL per 10⁶ cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Bovine, Horse, Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A bacterial fusion protein from the C-terminus of CENP-F |
| Conjugate | DyLight™ 650 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Liquid |
| Concentration | 0.60 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Sodium borate |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11155366 |
Target Information
This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2, the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitosis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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