Thermo Fisher Scientific HDAC9 Monoclonal Antibody (OTI6E2), TrueMAB
HDAC9 단백질을 타겟으로 하는 Mouse Monoclonal Antibody (Clone OTI6E2). Western Blot과 IHC(Paraffin)에 적합하며, 고순도의 Affinity Chromatography 정제 항체. PBS/BSA/Glycerol buffer에 보관되며 -20°C에서 안정적 저장 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6E2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 181–460 of human HDAC9 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Target Information
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is a critical component of transcriptional regulation, and acetylation of nucleosomal histones is a major source of this remodeling. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in nucleosomal conformation, increasing accessibility to transcription factors.
Several mammalian proteins function as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Conversely, histone deacetylation is associated with transcriptional silencing. The histone deacetylases (HDAC) family includes HDAC1–9. HDAC9 and HDAC9a are alternatively spliced isoforms; HDAC9a is 132 amino acids shorter but retains catalytic activity and represses myocyte enhancer-binding factor 2-mediated transcription. Both isoforms are expressed in brain, skeletal muscle, kidney, placenta, and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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