Thermo Fisher Scientific PARP1 Polyclonal Antibody
PARP1 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, ICC/IF, ELISA에 사용 가능. Bovine, Human, Mouse, Rat에 반응하며, 116 kDa PARP 및 85 kDa 절단 산물을 검출. 세포 사멸 연구 및 단백질 분석용 연구 전용 시약.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | – | View 1 publication |
| ELISA | Assay-dependent | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to aa 509–524 of bovine PARP auto modification domain |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2160723 |
Product Specific Information
By Western blot, this antibody detects a 116 kDa protein representing PARP and the 85 kDa apoptosis-induced cleavage product of protease resembling ICE (prICE) and CPP32 from GH4C1 cell extract.
Target Information
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyze the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85 kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Asp216 and Gly217, a site in PARP conserved across species.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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