Thermo Fisher Scientific PARP1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to aa 509-524 of bovine PARP auto modification
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2160723

Product Specific Information

By Western blot, this antibody detects a 116 kDa protein representing PARP and the 85 kDa apoptosis-induced cleavage product of protease resembling ICE (prICE) and CPP32 from GH4C1 cell extract.

Target Information

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.