Thermo Fisher Scientific Phospho-Vimentin (Ser56) Polyclonal Antibody
Phospho-Vimentin (Ser56) 특이적 항체로 Western blot, Flow cytometry, ELISA에 사용 가능. Rabbit polyclonal IgG 형식이며 Human, Mouse, Rat 반응성. Protein A 정제, 1 mg/mL 농도, -20°C 보관. 세포 내 단백질 수송 및 세포골격 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human Vimentin around the phosphorylation site of Ser56 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin, expressed along with desmin during early cellular development, is later replaced by tissue-specific IFPs. It plays a role in steroid synthesis by acting as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin leads to intermediate filament breakdown and cytoskeletal reorganization, facilitating lipid droplet interaction with mitochondria and enhancing steroid synthesis.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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