Thermo Fisher Scientific GADD45B Polyclonal Antibody
다중 종에 반응하는 Rabbit Polyclonal GADD45B 항체로, WB, IHC, ELISA, IP에 사용 가능. 합성 펩타이드 기반 면역원으로 높은 특이성과 재현성을 제공. 액상 형태로 -20°C 보관, 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 100–160 on mouse GADD45B protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Cell cycle progression is subject to arrest at G1 and G2 checkpoints in response to DNA damage, allowing time for DNA repair prior to S and M phase entry. The p53 tumor suppressor regulates one such G1 checkpoint and upregulates GADD45 and p21 expression.
GADD45 binds Cdks and PCNA, stimulating DNA excision repair in vitro and inhibiting S phase entry. It may serve as a link between the p53-dependent cell cycle checkpoint and DNA repair.
GADD45-like proteins, including GADD45 beta and GADD45 gamma, are induced by environmental stresses and activate p38/JNK via MEKK4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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