Thermo Fisher Scientific RAD52 Polyclonal Antibody
Human RAD52 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot 및 Immunocytochemistry에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS 기반 완충액에 보존됩니다. 단백질 손상 복구 연구용으로 사용됩니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human RAD52. Recombinant protein control fragment (Product #RP-105801) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2662872 |
Product Specific Information
Immunogen sequence:
ILGGRDSHPAA GGGSVLCFGQ CQYTAEEYQA IQKALRQRLG PEYISSRMAG GGQKVCYIEG HRVINLANEM FGYNGWAHSI TQQNVDFVDL NNGKFYVGVC AFVRVQLKDG SYHEDVGYGV SE
Highest antigen sequence identity to the following orthologs:
- Mouse: 90%
- Rat: 90%
Target Information
DNA double-strand breaks can be generated by exposure to ionizing radiation or through endogenous cellular reactions. These breaks are generally repaired via two mechanisms: homologous recombination (HR) or non-homologous end joining (NHEJ).
Homologous recombination requires the activity of RAD52 epistasis group members, including RAD50, RAD51, RAD52, RAD54, RDH54/TID1, RAD55, RAD57, RAD59, MRE11, and XRS2.
RAD52 catalyzes strand annealing reactions and forms oligomers that attach to ends of single-stranded DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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