Thermo Fisher Scientific cKit Polyclonal Antibody, Biotin
c-Kit 단백질을 인식하는 Biotin 결합 토끼 폴리클로날 항체. Western blot, IHC, ELISA, IP 등 다양한 응용에 적합. Affinity chromatography로 정제되었으며, 0.5–1.5 mg/mL 농도의 액상 형태. For Research Use Only.
- 카탈로그번호
- CKIT-112-BIOTIN
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Non-human primate, Pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide taken within amino acid region 430–480 on human Tyrosine-Kinase c-Kit protein |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT and its ligand regulate growth and activation of various hematopoietic and non-hematopoietic cells.
Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Deregulation of the KIT receptor tyrosine kinase, particularly the D816V activation loop mutation, is a therapeutic target in neoplastic mast cell growth.
c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and gastrointestinal tract Cajal cells. Upon binding of its ligand stem cell factor (SCF), c-Kit dimerizes, leading to receptor activation and autophosphorylation of tyrosine residues, including tyrosine 703 on the cytoplasmic domain. This enables docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-Kit can also activate PI3K, PLC-γ, and JAK/STAT pathways. c-Kit receptor activation is essential for hematopoiesis, stem cell maintenance, and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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