Thermo Fisher Scientific F(ab`)2-Donkey anti-Sheep IgG (H+L) Secondary Antibody, Biotin
Biotin 결합 F(ab`)2 Donkey anti-Sheep IgG (H+L) 2차 항체로, 높은 특이성과 민감도를 제공. ELISA, ICC/IF, Flow Cytometry 등 다양한 면역실험에 사용 가능. Affinity Chromatography로 정제되어 안정적인 신호 증폭 및 정확한 검출에 적합.
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Thermo Fisher Scientific F(ab`)2-Donkey anti-Sheep IgG (H+L) Secondary Antibody, Biotin
Applications 및 Tested Dilution
| Application | Dilution Range |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Sheep |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536033 |
| Target | IgG |
| Antibody Form | F(ab`)2 Fragment |
Additional Formats
Product Specific Information
The sensitivity of each lot of antibody is confirmed using ELISA.
The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).
Target Information
Anti-Sheep secondary antibodies are affinity-purified antibodies with well-characterized specificity for ovine (sheep) immunoglobulins and are useful in the detection, sorting, or purification of their specified target.
Secondary antibodies offer increased versatility, enabling use with many detection systems (e.g., HRP, AP, fluorescence).
They provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Most commonly, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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