Thermo Fisher Scientific Goat anti-Guinea Pig IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 680
DyLight 680 형광 표지된 염소 유래 항-기니피그 IgG(H+L) 2차 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. 교차흡착 처리로 비특이적 반응 최소화. 676/705 nm 형광 특성으로 적외선 이미징에 적합.
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Applications and Tested Dilutions
| Application | Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:2,000 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Guinea pig |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Guinea pig IgG-heavy and light chain |
| Conjugate | DyLight™ 680 |
| Excitation/Emission Max | 676/705 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556678 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, horse, human, mouse, rabbit, rat, and sheep |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to bovine, chicken, horse, human, mouse, rabbit, rat, and sheep.
Target Information
Anti-Guinea Pig secondary antibodies are affinity-purified antibodies with well-characterized specificity for guinea pig immunoglobulins. They are useful in the detection, sorting, or purification of guinea pig IgG targets. Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification, as multiple secondary antibodies can bind to a single primary antibody. These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to ensure high specificity.
Additional Formats
- DyLight 350
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 650
- DyLight 755
- DyLight 800
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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