Thermo Fisher Scientific eIF4A1 Polyclonal Antibody
eIF4A1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC, IP 등 다양한 응용에 사용 가능. Human, Mouse, Rat에 반응하며, 항원 친화 크로마토그래피로 정제된 고순도 제품. 연구용으로만 사용.
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Thermo Fisher Scientific eIF4A1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
| RNA Immunoprecipitation (RIP) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–295 of EIF4A1 (Uniprot ID: P60842) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2552078 |
Product Specific Information
- Recommended positive control: HepG2
- Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (96%), Dog (100%), Pig (100%), Chimpanzee (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
eIF4A2 is a 407 amino acid ATP-dependent RNA helicase belonging to the DEAD box helicase family. It contains one helicase ATP-binding domain and a helicase C-terminal domain.
eIF4A2, a subunit of the eIF4F complex, acts as a protein-synthesis initiation factor and plays an important role in the binding of mRNA to the 43S pre-initiation complex of ribosomes.
Human eIF4A2 interacts with the NS5B protein of Hepatitis C virus (HCV) and facilitates genomic RNA synthesis by unwinding the secondary structure of the HCV genome.
It is ubiquitously expressed in most normal tissues, but in variable amounts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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