Thermo Fisher Scientific Cullin 9 Polyclonal Antibody
Rabbit polyclonal antibody recognizing human Cullin 9 (PARC). Suitable for WB and IHC (1:300–1:2,000). Lyophilized form, unconjugated, stored at 4°C or -20°C. Used to study p53 localization and PARC function in tumor suppression.
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Applications
Western Blot (WB)
- Tested Dilution: 1:300–1:2,000
- Publications: View 5 publications
Immunohistochemistry (IHC)
- Tested Dilution: 1:300–1:2,000
- Publications: View 5 publications
Miscellaneous PubMed (Misc)
- Publications: View 5 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from internal region of human PARC (Cullin 9) conjugated to an immunogenic carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity: PARC.
Target Information
The continued localization of p53 to the nucleus is essential for its function as a tumor suppressor. PARC, a large Parkin-like ubiquitin ligase, acts as a cytoplasmic anchor protein in p53-associated complexes.
Inactivation of PARC leads to nuclear localization of p53 and activation of p53-dependent apoptosis, while overexpression promotes cytoplasmic sequestration of p53.
PARC knockout mice are viable with no obvious phenotype, suggesting functional redundancy among cullin family E3 ubiquitin ligases.
p53 binding to PARC may regulate PARC function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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