Thermo Scientific™

Pierce™ Sulfo-SANPAH, No-Weigh™ Format

Thermo Scientific Pierce Sulfo-SANPAH is a hetero-bifunctional crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide.자세히 알아보기

Thermo Scientific Pierce Sulfo-SANPAH is a hetero-bifunctional crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide. NHS esters react efficiently with primary amino groups (-NH2) in pH 7–9 buffers to form stable amide bonds. The reaction results in the release of sulfo-N-hydroxy-succinimide. When exposed to UV light, nitrophenyl azides form a nitrene group that can initiate addition reactions with double bonds, insertion into C-H and N-H sites, or subsequent ring expansion to react with a nucleophile (e.g., primary amines). Sulfo-SANPAH, supplied as a sodium salt, is useful for cell-surface protein crosslinking and possesses charged groups for water solubility to a concentration of 10 mM.

Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging prevents the loss of reagent reactivity and contamination over time by eliminating the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.

Features of Sulfo-SANPAH:

• Reactive groups: sulfo-NHS ester and nitrophenyl azide
• Reactive towards: amino groups and any nucleophile
• Non-cleavable
• N-Sulfosuccinimidyl-6-(4-azido-2-nitrophenylamino) hexanoate
• Optimal photolysis occurs at 320-350 nm; minimizes damage to biomolecules by irradiation
• Water soluble; non-cleavable

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

사양

화학물질 반응성Amine-Nonselective

CleavableBy Thiols

분자량492.4

페길레이티드No

스페이서 암 길이18.2 Å

세포 투과성No

배송 조건Ambient

제품라인Pierce™

라벨링 방법Chemical Labeling

교차결합제 유형Heterobifunctional

반응성 부분Sulfo-NHS Ester, Aryl Azide

스페이서Medium (10 to 30 Å)

형태Powder

수량50 mg

용해도Water

형식Standard, Single-use

수용성Yes

제품 유형Crosslinker

Unit SizeEach