Thermo Fisher Scientific RyR1 Monoclonal Antibody (G-1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:100

-

Immunocytochemistry (ICC/IF)

1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

1:500

-

ELISA (ELISA)

1:1,000

-

Product Specifications

Species Reactivity

Bovine, Dog, Human, Mouse, Pig, Rat

Published species

Human, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

G-1

Immunogen

Short synthetic polypeptide corresponding to the C-terminal domain of the ryanodine receptor.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2183056

Product Specific Information

For immunofluorescent applications, visualization by confocal microscopy is required. Detection by standard fluorescent microscopy will not be adequate to detect the RyR. Additionally, fluorescent, and not enzymatic, detection is required. Due to the intensity of confocal lasers, the use of an anti-fading agent such as DABCO is strongly recommended.

Mouse anti Human Ryanodine Receptor antibody, clone RYR-1 recognizes the human ryanodine receptor 1, also known as Skeletal muscle calcium release channel or skeletal muscle ryanodine receptor.

Target Information

Ryanodine receptors have been shown to play critical roles in the intracellular Ca2+ signaling occurring during cell activation in muscle cells and non-muscled cells. Ryanodine receptor, ~500 kDa, can be isolated from a variety of cell types such as lymphocytes, macrophages, granulocytes, fibroblasts, epithelial, endothelial cells, skeletal muscles, cardiac muscle and brain tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.