Thermo Fisher Scientific RyR1 Monoclonal Antibody (G-1)
RyR1 단백질 검출용 마우스 모노클로날 항체로, Western blot, IHC, ICC/IF, Flow cytometry, ELISA 등에 사용 가능. 다양한 종에 반응하며, 고순도 정제 및 PBS 버퍼 내 보존. 근육 세포 내 칼슘 신호 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:100 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | 1 publication |
| Flow Cytometry (Flow) | 1:500 | - |
| ELISA | 1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Human, Mouse, Pig, Rat |
| Published Species | Human, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | G-1 |
| Immunogen | Short synthetic polypeptide corresponding to the C-terminal domain of the ryanodine receptor |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2183056 |
Product Specific Information
- For immunofluorescent applications, visualization by confocal microscopy is required.
- Detection by standard fluorescent microscopy is not adequate for RyR detection.
- Fluorescent, not enzymatic, detection is required.
- Due to the intensity of confocal lasers, use of an anti-fading agent such as DABCO is strongly recommended.
- Mouse anti-Human Ryanodine Receptor antibody, clone RYR-1, recognizes the human ryanodine receptor 1 (Skeletal muscle calcium release channel).
Target Information
Ryanodine receptors play critical roles in intracellular Ca²⁺ signaling during cell activation in both muscle and non-muscle cells.
The ryanodine receptor (~500 kDa) can be isolated from various cell types, including lymphocytes, macrophages, granulocytes, fibroblasts, epithelial and endothelial cells, skeletal and cardiac muscles, and brain tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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