Thermo Fisher Scientific V5 Tag Polyclonal Antibody, Agarose

Applications

Tested Dilution

Publications

Immunoprecipitation (IP)

15 - 25 µL gel slurry/0.1 - 1 mg of protein lysate

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Product Specifications

Species Reactivity

Tag

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

V5 (GKPIPNPLLGLDST) conjugated to KLH.

Conjugate

Agarose Agarose Agarose

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 6.8 to 7.4

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Goats were immunized with V5 (GKPIPNPLLGLDST) conjugated to KLH.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.