Thermo Fisher Scientific Hot Start Taq Monoclonal Antibody (5G12)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

5G12

Immunogen

Taq DNA polymerase

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

5 U/µL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The Hot Start Taq Antibody is a mouse monoclonal antibody that specifically binds to Taq DNA polymerase. When this antibody is bound to Taq, the enzyme is rendered inactive. It provides an antibody-mediated hot start that enhances the specificity and sensitivity of PCR. Inhibition is completely reversed when the temperature is raised during the first denaturing step of thermal cycling.

Target Information

Used in the PCR method because of its high thermostability. Has a relatively high error rate because it lacks exonuclease proofreading functionality [Uniprot].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.