Thermo Fisher Scientific Hexanoyl-Lysine adduct Monoclonal Antibody (5D9), APC
Hexanoyl-Lysine 변형 단백질 검출용 APC 결합 단일클론 항체. Western blot, IHC, ICC, Flow cytometry, ELISA에 사용 가능. 화학적 반응성 종에 특이적이며, 보존제 무첨가 액상 형태로 제공. 4°C 보관, Protein G 정제.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:25–1:100 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5D9 |
| Immunogen | Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932231 |
Available Formats
- Unconjugated (MA5-27554)
- FITC (MA5-45778)
- PE (MA5-45780)
- PerCP (MA5-45779)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45777 was sufficient for detection of Hexanoyl Lysine adduct in 0.5 µg of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine.
Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
Hexanoyl-lysine (HEL) is a lipid peroxidation biomarker derived from the oxidation of omega-6 unsaturated fatty acids. HEL may serve as a biomarker for omega-6 PUFA oxidation in living organisms.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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