Thermo Fisher Scientific Rat IgG2a Isotype Control, Alexa Fluor 488

Applications

Tested Dilution

Control (Ctrl)

Assay-Dependent

Flow Cytometry (Flow)

Assay-Dependent

Product Specifications

Host/Isotype

Rat / IgG2a

Class

Control

Type

Isotype Control

Immunogen

Rat IgG2a

Conjugate

Alexa Fluor® 488 Alexa Fluor® 488 Alexa Fluor® 488

Excitation/Emission Max

View spectra

Form

liquid

Concentration

100 µg/mL

Purification

purified

Storage buffer

PBS with 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Blue

RRID

AB_2556535

Target Information

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.