Merck Amicon Ultra-0.5 Centrifugal Filter Unit 100 kDa
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카탈로그 번호 | 설명 | 상태 | 단위 | 가격 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
UFC510024 | Merck UFC510024 Ultracel-100 regenerated cellulose membrane, 0.5 mL sample volume 24/pk | 재고문의 | 24/pk | 0원 | 0원 | |
UFC510096 | Merck UFC510096 Ultracel-100 regenerated cellulose membrane, 0.5 mL sample volume 96/pk | 재고문의 | 96/pk | 0원 | 0원 | |
UFC5100BK | Merck UFC5100BK Ultracel-100 regenerated cellulose membrane, 0.5 mL sample volume 500/pk | 재고문의 | 500/pk | 0원 | 0원 |
material | polypropylene cap polypropylene tube regenerated cellulose membrane styrene-butadiene housing |
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무균 | non-sterile |
제품 라인 | Amicon® |
manufacturer/tradename | Amicon® Ultra |
파라미터 | 0.5 mL sample volume. |
technique(s) | protein extraction: suitable protein purification: suitable |
길이 | 4.99 cm (1.96 in.) |
직경 | 10.8 mm |
여과 면적 | 1 cm2 |
공극 크기 | 100 kDa NMWCO |
일반 설명
Amicon® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, IgG (156 kDa) at 1 mg/ml initial concentration is typically retained at >95% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
애플리케이션
- Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel® membrane
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
- Desalting, buffer exchange, and protein dialysis
특징 및 장점
- Ultracel® regenerated cellulose membrane with 3 kDa NMWCO delivers retentate recovery of greater than 90%
- Concentrates 500 μL down to 15 μL
- Concentration factor 25x to 30x
- High sample recovery (greater than 90% of dilute starting solution)
- Fast processing time: 10 to 30 minutes
- Reverse spin capability provides consistent recoveries
물리적 형태
Color Code: Clear
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