Thermo Fisher Scientific Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP

Applications

Tested Dilution

Western Blot (WB)

1:500-1:20,000

Immunohistochemistry (IHC)

1:500-1:20,000

ELISA (ELISA)

1:500-1:20,000

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

HRP HRP HRP

View additional formats

• [Alexa Fluor 488 ](/antibody/secondary/product-name/Human IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 488/host/Goat/)
• [Alexa Fluor 546 ](/antibody/secondary/product-name/Human IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 546/host/Goat/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Human IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 555/host/Goat/)
• [Alexa Fluor 568 ](/antibody/secondary/product-name/Human IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 568/host/Goat/)
• [Alexa Fluor 594 ](/antibody/secondary/product-name/Human IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 594/host/Goat/)
• [Alexa Fluor 633 ](/antibody/secondary/product-name/Human IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 633/host/Goat/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Human IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 647/host/Goat/)
• [Alexa Fluor Plus 405 ](/antibody/secondary/product-name/Human IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor Plus 405/host/Goat/)
• [Alexa Fluor Plus 488 ](/antibody/secondary/product-name/Human IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor Plus 488/host/Goat/)
• [Alexa Fluor Plus 555 ](/antibody/secondary/product-name/Human IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor Plus 555/host/Goat/)
• [Alexa Fluor Plus 594 ](/antibody/secondary/product-name/Human IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor Plus 594/host/Goat/)
• [Alexa Fluor Plus 647 ](/antibody/secondary/product-name/Human IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor Plus 647/host/Goat/)
• [AP](/antibody/secondary/product-name/Human IgG (H--L) Cross-Adsorbed/conjugates/AP/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Human IgG (H--L) Cross-Adsorbed/conjugates/Biotin/host/Goat/)
• [FITC](/antibody/secondary/product-name/Human IgG (H--L) Cross-Adsorbed/conjugates/FITC/host/Goat/)
• [Rhodamine](/antibody/secondary/product-name/Human IgG (H--L) Cross-Adsorbed/conjugates/Rhodamine/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Human IgG (H--L) Cross-Adsorbed/conjugates/TRITC/host/Goat/)
• Request custom conjugation

Form

Lyophilized

Concentration

2.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.1% ProClin 150

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2535588

Target

IgG

Cross Adsorption

Against bovine, mouse and rabbit serum proteins

Antibody Form

Whole Antibody

Product Specific Information

Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid. Based on Immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum immunoglobulins, serum proteins from bovine, mouse or rabbit.

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.