Thermo Fisher Scientific Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
HRP 결합 염소 항-인간 IgG(H+L) 교차흡착 2차 항체로 Western blot, IHC, ELISA에 적합. 인간 IgG에 높은 특이성과 감도를 제공하며, 비특이적 반응 최소화. 친화 크로마토그래피로 정제된 동결건조 형태로 안정적 장기 보관 가능.
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Thermo Fisher Scientific Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2535588 |
| Target | IgG |
| Cross Adsorption | Against bovine, mouse and rabbit serum proteins |
| Antibody Form | Whole Antibody |
Product Specific Information
Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.)
Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Store lyophilized material at 2–8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin human serum immunoglobulins or serum proteins from bovine, mouse, or rabbit.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins.
They are useful in detection, sorting, or purification of their specified targets.
Secondary antibodies provide versatility for various detection systems (HRP, AP, fluorescence) and enhance sensitivity through signal amplification.
They are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation).
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 546
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 633
- Alexa Fluor 647
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- AP
- Biotin
- FITC
- Rhodamine
- TRITC
- Request custom conjugation
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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