Thermo Fisher Scientific Donkey anti-Rabbit IgG (H+L) Secondary Antibody, WesternDot 625

Applications

Tested Dilution

Western Blot (WB)

1:500

Product Specifications

Host/Isotype

Donkey / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Rabbit Immunoglobins

Conjugate

WesternDot™ 625 WesternDot™ 625 WesternDot™ 625

View additional formats

• [Alexa Fluor 488 ](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Alexa Fluor 488/host/Donkey/)
• [Alexa Fluor 594 ](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Alexa Fluor 594/host/Donkey/)
• [AP](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/AP/host/Donkey/)
• [Biotin ](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Biotin/host/Donkey/)
• [FITC](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/FITC/host/Donkey/)
• [HRP](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/HRP/host/Donkey/)
• [Qdot 525](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Qdot 525/host/Donkey/)
• [Qdot 565](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Qdot 565/host/Donkey/)
• [Qdot 625](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Qdot 625/host/Donkey/)
• [Qdot 655](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Qdot 655/host/Donkey/)
• [TRITC](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/TRITC/host/Donkey/)
• Request custom conjugation

Form

Liquid

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient

RRID

AB_2556788

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

WesternDot fluorescently labeled secondary antibodies deliver superior signal-to-noise ratios and exhibit comparable sensitivity to enhanced chemiluminescence (ECL)-based methods in western blot applications. No stripping and re-probing or multiple blots are required for multiple protein detection as commonly needed with ECL or colorimetric detection. Differently labeled WesternDot antibodies can be applied to a single blot for simultaneous detection of multiple proteins using standard gel or blot imaging platforms (a list of instruments that are compatible with WesternDot detection can be found in the user manual below). Our WesternDot antibodies utilize Qdot nanocrystals enhanced with VIVID technology, making them brighter than the original Qdot reagents. Additionally, they have reduced intensity differences between the different colors, simplifying multiplex applications.

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.