Invitrogen™

Dynabeads™ Epithelial Enrich

Dynabeads Epithelial Enrich contains 4.5-μm superparamagnetic beads coupled to a monoclonal mouse anti-human EpCAM antibody and is intended for isolation of circulating epithelial tumor cells (CTCs) from liquid biopsy samples.

Dynabeads Epithelial Enrich consist of 4.5-μm superparamagnetic beads coupled to a monoclonal mouse anti-human EpCAM antibody and are intended for isolation of circulating epithelial tumor cells (CTCs) from liquid biopsy samples. The sensitive isolation method provided by Dynabeads Epithelial Enrich is necessary given the low number of CTCs in liquid biopsy samples. The isolated CTCs remain attached to the beads throughout the process.

For downstream molecular or proteomic analysis, the cells can be directly lysed while still attached to the beads. RT-PCR can be performed for amplification and quantification of target nucleic acids. CTC isolation and any subsequent bead-based proteomic or molecular applications can be performed manually or automated using the KingFisher Apex purification system.

Benefits of Dynabeads Epithelial Enrich include:

• High sensitivity and specificity isolation of CTCs from liquid biopsy samples
• Fast and efficient isolation of viable CTCs
• Automation user guide and script available for high-throughput enrichment using the KingFisher Apex instrument

사양

세포 유형Tumor Cells, Epithelial Cells

제품 유형Antibody Coated Bead

포함5 mL Dynabeads coated with the monoclonal antibody BerEP4 against the human epithelial antigen, EpCAM

분리 기술Depletion, positive isolation for molecular applications

셀 수Processes ∼4 x 109 cells total

출력 생존력>95%

제품라인DYNAL™, Dynabeads™

순도 또는 품질 등급Research Grade

수량5 mL

반응성Human

샘플 종류PBMC, Blood

배송 조건Room Temperature

시작 물질 셀 수2 x 107 PBMCs per isolation

타겟 종Human

Unit SizeEach