Thermo Fisher Scientific Ep-CAM/CD326 (Rat) Monoclonal Antibody (GZ-20)
쥐(Ep-CAM/CD326) 항원을 인식하는 마우스 단클론 항체로, Western blot, ICC/IF, Flow cytometry, IP에 적합합니다. Ep-CAM 관련 세포 접착 및 종양 연구용으로 사용되며, 단백질 A/G로 정제된 액상 비결합형 항체입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 5–10 µL / 10^6 cells |
| Immunoprecipitation (IP) | 5–10 µL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GZ-20 |
| Immunogen | Rat small intestine cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Protein A/G |
| Storage Buffer | Tissue culture supernatant, pH 7.4 |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Human
Target Information
Ep-CAM (epithelial adhesion molecule, also known as epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent cell adhesion molecule and influences cell cycle, proliferation, and metabolism by inducing proto-oncogene c-myc and cyclins A and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression of Ep-CAM is associated with enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial lineage tumors.
Ep-CAM is localized on the basolateral surface of most simple epithelia and many carcinoma types, and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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