Thermo Fisher Scientific Phospho-SMAD2/SMAD3 (Thr8) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human SMAD3(Accession P84022), corresponding to amino acid residues around phosphorylated Thr8. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SMAD2/SMAD3,uniProtId:Q15796-1,ncbiNodeId:9606,antigenRange:8,antigenLength:467,antigenImageFileName:PA5-99378_SMAD2SMAD3_Q15796-1_Rabbit.svg,antigenImageFileNamePDP:PA5-99378_SMAD2SMAD3_Q15796-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:SMAD2/SMAD3,uniProtId:P84022-1,ncbiNodeId:9606,antigenRange:8,antigenLength:425,antigenImageFileName:PA5-99378_SMAD2SMAD3_P84022-1_Rabbit.svg,antigenImageFileNamePDP:PA5-99378_SMAD2SMAD3_P84022-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2818311

Product Specific Information

Antibody detects endogenous levels of Smad2/3 only when phosphorylated at Threonine 8.

Target Information

Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.