Thermo Fisher Scientific LIMS1 Polyclonal Antibody
LIMS1 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, ICC/IF, ELISA에 사용 가능. Human 및 Mouse 반응성. Affinity Chromatography로 정제된 액상 형태이며, PBS buffer(50% glycerol)로 보존. 세포 접착 및 신호전달 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–325 of human LIMS1 (NP_001180417.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.51 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805631 |
Product Specific Information
Immunogen sequence:
MANALASATC ERCKGGFAPA EKIVNSNGEL YHEQCFVCAQ CFQQFPEGLF YEFEGRKYCE HDFQMLFAPC CHQCGEFIIG RVIKAMNNSW HPECFRCDLC QEVLADIGFV KNAGRHLCRP CHNREKARGL GKYICQKCHA IIDEQPLIFK NDPYHPDHFN CANCGKELTA DARELKGELY CLPCHDKMGV PICGACRRPI EGRVVNAMGK QWHVEHFVCA KCEKPFLGHR HYERKGLAYC ETHYNQLFGD VCFHCNRVIE GDVVSALNKA WCVNCFACST CNTKLTLKNK FVEFDMKPVC KKCYEKFPLE LKKRLKKLAE TLGRK
Positive Samples: Mouse heart
Cellular Location: Cell junction, Cell membrane, Cytoplasmic side, Peripheral membrane protein, focal adhesion
Target Information
The protein encoded by this gene is an adaptor protein containing five LIM domains (double zinc fingers). It is likely involved in integrin signaling through LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It may act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Localization to the periphery of spreading cells suggests a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been identified.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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