Thermo Fisher Scientific SF1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1.25 µg/mL

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

4-8 µg/mL

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Product Specifications

Species Reactivity

Human

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the N-terminal of human SF1 (aa 6-55). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SF1,uniProtId:Q15637-1,ncbiNodeId:9606,antigenRange:6-55,antigenLength:639,antigenImageFileName:PA5-41967_SF1_Q15637-1_Rabbit.svg,antigenImageFileNamePDP:PA5-41967_SF1_Q15637-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2607741

Product Specific Information

Peptide sequence: NATPLDFPSK KRKRSRWNQD TMEQKTVIPG MPTVIPPGLT REQERAYIVQ

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%

Target Information

Splicing factor 1 is a nuclear pre-mRNA splicing factor. The protein specifically recognizes the intron branch point sequence at the 3` splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.