Thermo Fisher Scientific SF1 Polyclonal Antibody
Human SF1 단백질의 N-말단을 표적으로 하는 Rabbit Polyclonal Antibody로, WB 및 IHC(P)에서 검증됨. Protein A로 정제된 액상 형태이며, PBS + sucrose 버퍼에 보관. 다양한 종에서 높은 서열 상동성 보유.
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Thermo Fisher Scientific SF1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1.25 µg/mL | View 5 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 4–8 µg/mL | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide directed towards the N-terminal of human SF1 (aa 6–55) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 2% sucrose |
| Contains | 0.09% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2607741 |
Product Specific Information
Peptide sequence:
NATPLDFPSK KRKRSRWNQD TMEQKTVIPG MPTVIPPGLT REQERAYIVQ
Sequence homology:
Cow: 100%
Dog: 100%
Guinea Pig: 100%
Horse: 100%
Human: 100%
Mouse: 100%
Rabbit: 100%
Rat: 100%
Zebrafish: 86%
Target Information
Splicing factor 1 (SF1) is a nuclear pre-mRNA splicing factor. It specifically recognizes the intron branch point sequence at the 3′ splice site together with the large subunit of U2 auxiliary factor (U2AF), and is essential for early spliceosome assembly. SF1 also contributes to nuclear pre-mRNA retention and transcriptional repression. The protein includes:
- N-terminal U2AF ligand motif
- Central hnRNP K homology motif and quaking 2 region (bind branch-site adenosine)
- Zinc knuckles domain
- C-terminal proline-rich domain
Alternative splicing results in multiple transcript variants.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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