Norgen Urine Exosome and Free-Circulating RNA Isolation Midi Kit

Norgen Urine Exosome and Free-Circulating RNA Isolation Midi Kit

SKU: 59300
All-in-one system for the purification of exosomes and sequential isolation of RNA and free-circulating RNA from urine samples.

Features and Benefits

• Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
• Versatile sample input ranges
• Isolate all sizes of free-circulating RNA, including microRNA
• Bind and elute all RNA irrespective of size or GC content, without bias
• Purified exosomal RNA is free from any circulating RNA-binding proteins
• No phenol extractions, Proteinase K treatment, or carrier RNA required
• No ultracentrifugation, filtration, or special syringes required
• No precipitation reagents or overnight incubation required
• Flexible elution volume (50–100 µL)
• Purification based on spin column chromatography using Norgen’s proprietary resin matrix

Description

These kits provide a fast, reliable, and convenient method to sequentially isolate and concentrate exosomal RNA and free-circulating RNA from different urine sample volumes.
The purification is based on spin column chromatography employing Norgen’s proprietary resin.
All sizes of RNA, including microRNA, are isolated without bias and without special instrumentation or toxic reagents.
The purified RNA is suitable for downstream applications such as:

• Real-time PCR
• Reverse transcription PCR
• Northern blotting
• RNase protection and primer extension
• Expression array assays

Kit Versions

Kit Specifications

*Refer to product insert (page 5) for average yields and RNA quantification methods.

Storage Conditions and Product Stability

All buffers should be tightly sealed and stored at room temperature.
Kit stability: 2 years after shipment.
If salt precipitation is observed, warm Lysis Buffer A for 20 minutes at 60°C.

Important Note

Urine samples stored at -70°C, -20°C, or 4°C may develop precipitation due to protein aggregation, which can cause exosome loss and affect RNA quality.
Use Norgen’s Urine Preservative for optimal nucleic acid and protein preservation at ambient temperature.
Samples can be stored for over 2 years without degradation.

Kit Components

Supporting Data

Figure 3. Isolation of RNA from exosomes purified from different urine volumes.
Norgen’s Urine Exosome and Free-Circulating RNA Isolation Midi Kit (Cat# 59300) was used to isolate exosomal RNA from urine volumes ranging from 2 mL to 10 mL. Two microlitres of isolated RNA were used in RT-qPCR to assess urinary exosomal miR-30a amplification.
(A) The urinary exosomal miR-30a decreases linearly with increasing sample input volume.
(B) Relative amount shows excellent linearity with >90% recovery.

Figure 4. Isolation of Free-Circulating RNA after Exosomal RNA isolation.
Using the same kit, free-circulating RNA was isolated following exosomal RNA purification from urine volumes of 2–10 mL. Two microlitres of RNA were used in RT-qPCR for urinary free-circulating miR-21 amplification.
(A) The free-circulating miR-21 decreases linearly with increasing sample input volume.
(B) Relative amount shows excellent linearity with >90% recovery.

Documentation

Protocols

• (59300) Urine Exosome and Free-Circulating RNA Isolation Midi Kit - Protocol (25 prep)

Safety Data Sheets

• 59300 - Urine Exosome and Free-Circulating RNA Isolation Midi Kit - SDS

Related Products

• Urine Exosome Purification Mini Kit
• Urine Exosome Purification and RNA Isolation Mini Kit
• Exosomal RNA Isolation Kit

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