Norgen Urine Exosome and Free-Circulating RNA Isolation Midi Kit

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소변 시료에서 엑소좀 및 자유 순환 RNA를 순차적으로 분리하는 올인원 키트. 모든 크기의 RNA(마이크로RNA 포함)를 편향 없이 정제 가능. 초원심분리나 페놀 추출 불필요하며 간편한 스핀 컬럼 방식 사용. 2~10mL 시료 처리에 적합한 Midi 형.

카탈로그번호: 59300
브랜드: Norgen
카테고리: RNA Kits
판매단위: pk

#Norgen #Reagents #Exosome Reagents #R

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Norgen · Norgen Urine Exosome and Free-Circulating RNA Isolation Midi Kit

Norgen Urine Exosome and Free-Circulating RNA Isolation Midi Kit

SKU: 59300
All-in-one system for the purification of exosomes and sequential isolation of RNA and free-circulating RNA from urine samples.

Features and Benefits

Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
Versatile sample input ranges
Isolate all sizes of free-circulating RNA, including microRNA
Bind and elute all RNA irrespective of size or GC content, without bias
Purified exosomal RNA is free from any circulating RNA-binding proteins
No phenol extractions, Proteinase K treatment, or carrier RNA required
No ultracentrifugation, filtration, or special syringes required
No precipitation reagents or overnight incubation required
Flexible elution volume (50–100 µL)
Purification based on spin column chromatography using Norgen’s proprietary resin matrix

Description

These kits provide a fast, reliable, and convenient method to sequentially isolate and concentrate exosomal RNA and free-circulating RNA from different urine sample volumes.
The purification is based on spin column chromatography employing Norgen’s proprietary resin.
All sizes of RNA, including microRNA, are isolated without bias and without special instrumentation or toxic reagents.
The purified RNA is suitable for downstream applications such as:

Real-time PCR
Reverse transcription PCR
Northern blotting
RNase protection and primer extension
Expression array assays

Kit Versions

Kit Type	Sample Volume Range
Mini Kit	250 µL – 1 mL
Midi Kit	2 mL – 10 mL
Maxi Kit	11 mL – 30 mL

Kit Specifications

Specification	Description
Minimum Urine Input	2 mL
Maximum Urine Input	10 mL
Size of RNA Purified	All sizes, including miRNA and small RNA (<200 nt)
Elution Volume	50–100 µL
Time to Complete 10 Purifications	35–40 minutes
Average Yields*	Variable depending on specimen

*Refer to product insert (page 5) for average yields and RNA quantification methods.

Storage Conditions and Product Stability

All buffers should be tightly sealed and stored at room temperature.
Kit stability: 2 years after shipment.
If salt precipitation is observed, warm Lysis Buffer A for 20 minutes at 60°C.

Important Note

Urine samples stored at -70°C, -20°C, or 4°C may develop precipitation due to protein aggregation, which can cause exosome loss and affect RNA quality.
Use Norgen’s Urine Preservative for optimal nucleic acid and protein preservation at ambient temperature.
Samples can be stored for over 2 years without degradation.

Kit Components

Component	Cat. 59200 (Mini, 50 preps)	Cat. 59300 (Midi, 25 preps)	Cat. 59400 (Maxi, 15 preps)
Slurry E	12.5 mL	12.5 mL	12.5 mL
ExoC Buffer	8 mL	30 mL	50 mL
ExoR Buffer	12 mL	12 mL	12 mL
Lysis Buffer A	2 × 20 mL	2 × 20 mL	2 × 20 mL
Lysis Additive B	2 mL	2 mL	2 mL
Wash Solution A	2 × 18 mL	18 mL	18 mL
Elution Solution A	2 × 6 mL	6 mL	6 mL
Mini Filter Spin Columns	50	25	15
Mini Spin Columns	100	50	30
Collection Tubes	100	50	30
Elution Tubes (1.7 mL)	100	50	30
Product Insert	1	1	1

Supporting Data

Figure 3. Isolation of RNA from exosomes purified from different urine volumes.
Norgen’s Urine Exosome and Free-Circulating RNA Isolation Midi Kit (Cat# 59300) was used to isolate exosomal RNA from urine volumes ranging from 2 mL to 10 mL. Two microlitres of isolated RNA were used in RT-qPCR to assess urinary exosomal miR-30a amplification.
(A) The urinary exosomal miR-30a decreases linearly with increasing sample input volume.
(B) Relative amount shows excellent linearity with >90% recovery.

Figure 4. Isolation of Free-Circulating RNA after Exosomal RNA isolation.
Using the same kit, free-circulating RNA was isolated following exosomal RNA purification from urine volumes of 2–10 mL. Two microlitres of RNA were used in RT-qPCR for urinary free-circulating miR-21 amplification.
(A) The free-circulating miR-21 decreases linearly with increasing sample input volume.
(B) Relative amount shows excellent linearity with >90% recovery.