Thermo Fisher Scientific SSEA1 Monoclonal Antibody (MC-480), PE
SSEA1 (CD15) 항원을 인식하는 마우스 단클론 항체로, PE 형광체로 결합되어 있습니다. 인간 및 마우스 시료의 Flow Cytometry와 ICC/IF 분석에 적합합니다. 565/576 nm에서 여기/방출하며, 4°C에서 보관합니다.
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Applications
- Flow Cytometry (Flow)
Tested Dilution: 1:50–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MC-480 |
| Immunogen | BALB/c mouse immunized with F9 teratocarcinoma stem cells (X-irradiated) |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography – MBP |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536697 |
Additional Formats
- Unconjugated (MA1-022)
- DyLight 488 (MA1-022-D488)
- DyLight 550 (MA1-022-D550)
- DyLight 650 (MA1-022-D650)
- Custom conjugation available upon request
Product Specific Information
MA1-022-PE has been successfully used in ICC/IF and flow cytometry applications on mouse and human samples.
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on glycolipids, glycoproteins, and proteoglycans of various cell types such as granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system cells, and various tumor cells.
Variants include sialyl-Lewis X and sulphated Lewis X. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation based on calcium-dependent Le(x)-Le(x) interactions, relevant in embryo compaction and teratocarcinoma cell autoaggregation.
Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression is widely used to confirm diagnoses of Hodgkin’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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