Thermo Fisher Scientific SSEA1 Monoclonal Antibody (MC-480), PE

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:50-1:500

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

MC-480

Immunogen

BALB/c mouse immunized with F9 teratocarcinoma stem cells (X-irradiated). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SSEA1 (CD15),uniProtId:Q11127-1,ncbiNodeId:10090,antigenRange:1-433,antigenLength:433,antigenImageFileName:MA1-022-PE_SSEA1_CD15_Q11127-1_House_mouse.svg,antigenImageFileNamePDP:MA1-022-PE_SSEA1_CD15_Q11127-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

PE PE PE

View additional formats

• Unconjugated
• DyLight 488
• DyLight 550
• DyLight 650
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography - MBP

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536697

Product Specific Information

MA1-022-PE has been successfully used in ICC/IF and flow cytometry applications on mouse and human samples.

Target Information

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin`s disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.