Thermo Fisher Scientific PAF49 Polyclonal Antibody
Rabbit polyclonal antibody against human PAF49, suitable for WB, IHC, ICC, and IP applications. Recognizes residues 404–454 of RNA polymerase I-associated factor. Unconjugated liquid form, 0.20 mg/mL concentration, stored at 4°C. For research use only.
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Thermo Fisher Scientific PAF49 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 404 and 454 of human RNA polymerase I-associated factor PAF49 (CD3-epsilon-associated protein) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application notes:
- For ICC, formaldehyde fixation is recommended.
- Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
- For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Target Information
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Component of RNA polymerase I which synthesizes ribosomal RNA precursors.
Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.
Isoform 2 has been described as a component of the preformed T-cell receptor (TCR) complex.
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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