Thermo Fisher Scientific beta Lactamase Monoclonal Antibody (8A5.A10)

Applications

Tested Dilution

Publications

Western Blot (WB)

10 µg/mL

View 1 publication 1 publication

ELISA (ELISA)

10-20 µg/mL

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Product Specifications

Species Reactivity

Bacteria

Published species

Bacteria

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

8A5.A10

Immunogen

5`-His-tagged TEM-1 beta-lactamase.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.6 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1070669

Product Specific Information

MA1-10712 detects beta Lactamase from E. coli samples.

MA1-10712 has been successfully used in ELISA and Western blot applications.

The MA1-10712 immunogen is 5-His-tagged TEM-1 beta-lactamase.

Target Information

The beta lactam antibiotics (penicillins and cephalosporins) are the most frequently used antimicrobial agents. All of the beta lactams are structurally related through the presence of a core beta lactam ring. Bacterial resistance to beta lactams continues to increase, primarily due to the production of beta lactamases. Beta lactamases catalyze the hydrolysis of the beta lactam bond, which destroys antibacterial activity. Bacteria that produce TEM type or SHV type beta lactamases have point mutations in structural genes that have extended the substrate specificity of these beta lactamases. As a result, many of the beta lactamase producing Gram negative pathogens have become multidrug resistant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.