Thermo Fisher Scientific MRPS15 Polyclonal Antibody
Thermo Fisher Scientific의 MRPS15 Polyclonal Antibody는 인간 MRPS15 단백질을 인식하는 토끼 유래 항체로, Western blot, IHC, ICC/IF, IP에 적합합니다. 고순도의 항원 친화 크로마토그래피로 정제되었으며, 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Immunoprecipitation (IP) | 1–5 µL/mg of lysate |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived Human MRPS15 fragment |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography, Protein A |
| Storage Buffer | PBS, pH 7 |
| Contains | 0.03% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2902682 |
Target Information
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and are involved in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit, with an estimated 75% protein to rRNA composition, unlike prokaryotic ribosomes where this ratio is reversed. Prokaryotic ribosomes also contain a 5S rRNA, which is absent in mammalian mitoribosomes.
Among species, the proteins of the mitoribosome differ significantly in sequence and biochemical properties, complicating recognition by sequence homology. The MRPS15 gene encodes a 28S subunit protein belonging to the ribosomal protein S15P family. This protein is more than twice the size of its E. coli counterpart, with conserved 12S rRNA binding sites. Between human and mouse, MRPS15 is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to MRPS15 are found on chromosomes 15q and 19q.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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