Thermo Fisher Scientific N-Ras Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.3-1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (RMKKLNSSDDGTQ) corresponding to the internal amino acids of NRAS (aa 167-180). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:N-Ras,uniProtId:P01111-1,ncbiNodeId:9606,antigenRange:167-180,antigenLength:189,antigenImageFileName:PA5-18888_N-Ras_P01111-1_Goat.svg,antigenImageFileNamePDP:PA5-18888_N-Ras_P01111-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_10979909

Product Specific Information

This antibody is predicted to react with canine and mouse based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.

Target Information

NRAS (GTPase NRas) is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML) and Noonan syndrome 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.