Thermo Fisher Scientific 5-Methylcytosine Monoclonal Antibody (33D3)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:500

-

Dot blot (DB)

1:300

-

Methylated DNA Immunoprecipitation (MeDIP)

0.1 - 2 µg per IP

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

33D3

Immunogen

5-Methylcytosine nucleotide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.2 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2898346

Target Information

Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.