Thermo Fisher Scientific CD83 Recombinant Rabbit Monoclonal Antibody (104)

Applications

• Western Blot (WB): 1:500–1:2,000

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.

Advantages:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal-origin–free formulation
• Broad immunoreactivity due to the large rabbit immune repertoire

This antibody specifically recognizes human CD83.

Target Information

CD83 is a 40–45 kDa glycoprotein expressed on peripheral blood dendritic cells. Peripheral lymphocytes can express low levels of CD83 after stimulation with agents such as Con A or PHA. In immunohistology, CD83 is strongly expressed by interfollicular interdigitating reticulum cells and weakly by cells within germinal centers. It is also expressed by Langerhans cells in the skin.

The CD83 antigen is a 186-amino-acid single-chain glycoprotein belonging to the immunoglobulin superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane region, and a short cytoplasmic tail. It undergoes extensive post-translational glycosylation, resulting in a molecular weight approximately twice that of the predicted core protein.

CD83⁺ cells exhibit a unique immunophenotype distinct from T cells, B cells, NK cells, or myelomonocytic lineages, and coexpress high levels of MHC class II molecules along with markers such as CD2, CD5, CD40, CD78, CD13, CD33, CD36, and various cytokine receptors.

Diseases associated with CD83 dysfunction include plague and Rift Valley Fever.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.