Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (IT2.2), NovaFluor Blue 660-120S, eBioscience

Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (IT2.2), NovaFluor Blue 660-120S

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.2 µg)/test

---

Product Specifications

---

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 647, 700
• APC, Biotin
• Brilliant UV 737, Brilliant Violet 480, 711, 786
• Functional Grade
• NovaFluor Red 700, 725, 755
• NovaFluor Yellow 610, 690, 700, 755
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436, 600, 645
• Custom conjugation available

---

Product Specific Information

The IT2.2 monoclonal antibody reacts with human CD86 (B7-2), an ~80 kDa surface receptor. CD86 and CD80 are members of the B7 family of costimulatory molecules.
CD86 is expressed on B cells, macrophages, and dendritic cells and upregulated through stimuli such as BCR complex, CD40, and cytokine receptors.
It interacts with CD28 and CD152 (CTLA-4) on T cells, playing a key role in T-B crosstalk, T cell costimulation, and antibody production.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested with normal human peripheral blood cells at 5 µL (0.2 µg)/test.
Cell number range: 10⁵–10⁸ cells/test.

Note: NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for optimal results.

Blocking Buffer Recommendation:
Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for all experiments with NovaFluor conjugates.
Add 5 µL per sample before combining with cells or antibody cocktails.

NovaFluor conjugates utilize Phiton™ technology, offering engineered fluorescent signatures with minimized spillover and spread.

Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

---

Target Information

CD86 is a ligand for CTLA4 and CD28, acting as a costimulatory molecule in T-cell activation.
It is expressed by antigen-presenting cells and regulates immune responses through its interactions with T-cell surface proteins.
Alternative splicing produces multiple isoforms.
Associated diseases include gallbladder squamous cell carcinoma and myocarditis.

---

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

---

제품 이미지