Thermo Fisher Scientific HuC/HuD Monoclonal Antibody (16A11), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

Immunohistochemistry (IHC)

5-20 µg/mL

View 38 publications 38 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

-

View 9 publications 9 publications

ELISA (ELISA)

1-10 µg/mL

-

In Situ Hybridization (ISH)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human

Published species

Fish, Guinea pig, Human, Mouse, Pig, Rat, Zebrafish

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

16A11

Immunogen

Human HuC/HuD neuronal protein.

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• Request custom conjugation

Form

Lyophilized

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2535822

Product Specific Information

This antibody recognizes the Elav family members HuC, HuD and Hel-N1 neuronal proteins. It does not recognize HuR, another Elav family member that is present in all proliferating cells. The antibody has been shown to specifically label neuronal cells in zebrafish, chick, canaries, and humans, and is likely to label neuronal cells in most vertebrate species. Labeling is visible early in development, at about the time that the neurons leave the mitotic cycle.

Storage and reconstitution: Upon receipt, lyophilized antibodies should be stored desiccated at -20ºC or lower. When properly stored, these products are stable for at least one year. To prepare stock solution, reconstitute the antibody in 0.5-1 mL PBS, pH 7.4, containing 1% BSA. Store the solution for up to two weeks at 4ºC with the addition of 2mM sodium azide. For longer storage, divide solutions into single-use aliquots and freeze at -20ºC, avoidig freeze/thaw cycles.

For best results in immunohistochemistry, antigen retrieval is required. Use 50mM Tris, pH 8.0, for 30 minutes. The biotin-XX labeled antibody can be detected by using fluorescently labeled avidin, streptavidin or NeutrAvidin conjugates.

Target Information

Anti-Hu antibodies were originally isolated from a patient with paraneoplastic encephalomyelitis, a rare condition of selective neural tissue injury associated with small cell lung carcinoma. The antibodies bind specifically to antigens present exclusively in neuronal cells and are thus useful as markers of neuronal cells in tissue. The Hu antigen is an RNA-binding protein of the embryonic lethal abnormal visual (Elav) family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.