Takara RACE-ready human cDNA: brain & CNS

Overview

• Adaptor-ligated and ready for RACE PCR
• Tissue-specific pool of double-stranded cDNA
• Obtain full-length cDNAs without constructing or screening a cDNA library
• Ideal template for Marathon cDNA Amplification

Applications

• Rapid amplification of cDNA ends (RACE)
• Study of tissue-specific gene expression
• Discovery of polymorphic forms of mRNA
• Obtain full-length copies of published cDNAs
• Characterization of tissue-specific expression patterns

Source

For current source information, please refer to the Certificate of Analysis accompanying each Marathon-Ready cDNA.

References

• Chenchik, A., et al. Full-length cDNA cloning and determination of mRNA 5′ and 3′ ends by amplification of adaptor-ligated cDNA. BioTechniques 21, 526–532 (1996).
• Chenchik, A., Moqadam, F., Siebert, P. Marathon cDNA amplification: a new method for cloning full-length cDNAs. Clontechniques 1, 5–8 (1995).
• Borson, N. D., Salo W. L., Drewes, L. R. A lock-docking oligo(dT) primer for 5′ and 3′ RACE PCR. PCR Methods Appl. 2, 144–148 (1992).

Additional Product Information

Please refer to the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications.
Kit Components Lists and Certificates of Analysis are available under the Documents tab.