Takara RACE-ready human cDNA: brain & CNS

Overview

• Adaptor-ligated and ready for RACE PCR
• Tissue-specific pool of double-stranded cDNA
• Obtain full-length cDNAs without ever constructing or screening a cDNA library
• Perfect template for Marathon cDNA Amplification

More Information

Applications

• Rapid amplification of cDNA ends (RACE)
• Study tissue-specific gene expression
• Discover polymorphic forms of mRNA
• Obtain full-length copies of published cDNAs
• Characterization of tissue-specific patterns of gene expression

Source

For current source information, please refer to the Certificate of Analysis accompanying each Marathon-Ready cDNA.

References

Chenchik, A., et al. Full-length cDNA cloning and determination of mRNA 5′ and 3′ ends by amplification of adaptor-ligated cDNA. BioTechniques 21, 526–532 (1996).

Chenchik, A., Moqadam, F., Siebert, P. Marathon cDNA amplification: a new method for cloning full-length cDNAs Clontechniques 1, 5–8 (1995).

Borson, N. D., Salo W.L., Drewes, L.R. A lock-docking oligo(dT) primer for 5and 3 RACE PCR. PCR Methods Appl. 2, 144–148 (1992).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.