Thermo Fisher Scientific XRCC4 Monoclonal Antibody (OTI4H9)
Western blot, IHC, ICC/IF에 적합한 XRCC4 단일클론 항체로 Human 및 Mouse 시료에 반응. Affinity chromatography로 정제된 액상 제품이며, 안정적인 PBS buffer에 보존. DNA 복구 및 V(D)J 재조합 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4H9 |
| Immunogen | Full-length protein expressed in 293T cells transfected with human XRCC4 expression vector |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2725686 |
Target Information
The x-ray repair cross-complementing (XRCC) proteins are responsible for efficiently repairing and maintaining genetic stability following DNA base damage. These genes share sequence similarity with the yeast DNA repair protein Rad51.
XRCC4 is an accessory factor of DNA ligase IV that preferentially binds DNA with nicks or broken ends. It enhances the joining activity of DNA ligase IV and is involved in V(D)J recombination.
Defects in components of the DNA repair/V(D)J recombination machinery (Ku-70, Ku-80, DNA-PKCS, XRCC4, DNA ligase IV) can cause failure in V(D)J rearrangement and early block in T and B cell maturation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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