Invitrogen™

RiboMinus™ Eukaryote Kit v2

The RiboMinus™ Eukaryote Kit v2 provides truly complete transcriptome isolation for next generation sequencing analysis by offering selective depletion of자세히 알아보기

The RiboMinus™ Eukaryote Kit v2 provides truly complete transcriptome isolation for next generation sequencing analysis by offering selective depletion of up to 99.9% of the 5S, 5.8S, 18S, and 28S ribosomal RNA from total RNA versus poly-A or cap binding methods, which introduce bias and select for only part of the transcriptome.

Features of the kit include:

• Seamless integration with RiboMinus™Concentration Module (available separately) to purify RNA after ribosomal RNA depletion
• 1.5-hour workflow
• Input range of 1-5 μg

The RiboMinus™ Eukaryote Kit v2 improves upon the previous system (RiboMinus™ Eukaryote Kit for RNA-Seq) by removing significantly more rRNA (both cytoplasmic and mitochondrial) from the total RNA. When used with our library construction kits for sequencing, the RiboMinus™ Eukaryote Kit v2 provides maximum mapping to reference database with minimum bias, maintaining relative gene expression levels of even smaller RNA species. The resulting total RNA after depletion contains minimum contaminating rRNA with reduced variability.

The RiboMinus Eukaryote Kit v2 is designed for the removal of human, mouse, and rat rRNA. The RiboMinus™ Concentration Module (available separately) is used for RNA purification after rRNA depletion. For magnetic bead-based purification, please see the RiboMinus™ Eukaryote System v2. The RiboMinus Eukaryote Kit v2 has an RNA input range of 1-5 μg. For lower inputs (down to 100 ng), the Low Input RiboMinus™ Eukaryote System v2 is recommended.

The RiboMinus™ Eukaryote Kit v2 contains sufficient reagents to perform 12 ribosomal RNA depletions.

사양

Isolation TechnologyMagnetic Bead

종Human, Mouse, Rat

최종 제품 유형Transcriptome RNA

용도(장비)Ion PGM™ System, Ion Proton™ System, Illumina™ Genome Analyzer II (GA II)

제품라인RiboMinus™

수량12 reactions

시퀀싱 유형Transcriptome Sequencing

시작 물질 양1 - 5 μg total RNA

워크플로우 단계Sample Enrichment

Unit SizeEach