Thermo Fisher Scientific MID1 Polyclonal Antibody
MID1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, ICC/IF, ELISA에 적합합니다. 인간 및 생쥐 반응성을 가지며, 고순도 친화 크로마토그래피 정제 제품입니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific MID1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 478–667 of human MID1 (NP_000372.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.54 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2855368 |
Product Specific Information
Immunogen sequence:
KLKTNSQPFK LDPKSAHRKL KVSHDNLTVE RDESSSKKSH TPERFTSQGS YGVAGNVFID SGRHYWEVVI SGSTWYAIGL AYKSAPKHEW IGKNSASWAL CRCNNNWVVR HNSKEIPIEP APHLRRVGIL LDYDNGSIAF YDALNSIHLY TFDVAFAQPV CPTFTVWNKC LTIITGLPIP DHLDCTEQLP
Target Information
Midline-1 (Tripartite motif-containing protein 18, Putative transcription factor XPRF, RING finger protein 59) is a 667 amino acid protein encoded by the human gene MID1.
Midline-1 belongs to the TRIM/RBCC family and contains:
- Two B box-type zinc fingers
- One B30.2/SPRY domain
- One COS domain
- One fibronectin type-III domain
- One RING-type zinc finger
Midline-1 is believed to have E3 ubiquitin ligase activity, targeting the catalytic subunit of protein phosphatase 2 for degradation.
It is a cytoplasmic protein found as a homodimer or heterodimer with Midline-2 and interacts with IGBP1 (Lymphocyte signaling protein A4).
Defects in MID1 cause Opitz syndrome type I (OS-I), an X-linked recessive disorder characterized by hypertelorism, genital-urinary defects, lip-palate-laryngotracheal clefts, imperforate anus, developmental delay, and congenital heart defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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