Thermo Fisher Scientific DYNC1I2 Polyclonal Antibody
Rabbit polyclonal antibody against human DYNC1I2. Validated for WB, IHC, and ICC/IF. High specificity with 89% sequence identity to mouse and rat orthologs. Suitable for research use only; supplied as liquid form with antigen affinity purification.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human DYNC1I2. Recombinant protein control fragment (Product #RP-103172). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2640846 |
Product Specific Information
Immunogen sequence:
EDDDVVAPKP PIEPEEEKTL KKDEENDSKA PPHELTE
Ortholog sequence identity:
- Mouse: 89%
- Rat: 89%
Target Information
Dyneins are multi-subunit, high molecular weight ATPases that interact with microtubules to generate force by converting ATP’s chemical energy into mechanical movement. They consist of heavy, intermediate, light, and light-intermediate chains that form minus end-directed motor complexes transporting cellular cargos toward the cell center.
Axonemal Dynein motors, containing one to three non-identical heavy chains, enable microtubule sliding in cilia and flagella, essential for motility. Cytoplasmic Dynein is a 12-subunit complex with heavy, intermediate, and light chains, performing critical roles like organelle transport and centrosome assembly. The carboxy-terminus of Dynein is highly conserved and vital for microtubule-dependent motility, while the amino-terminal regions are more variable. Regulatory proteins such as Dynactin, LIS1, and nudel (nudE-like) modulate Dynein activity.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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