Thermo Fisher Scientific N-cadherin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 500-800 of human N-Cadherin (NP_0017832) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:N-cadherin,uniProtId:P19022-1,ncbiNodeId:9606,antigenRange:500-800,antigenLength:906,antigenImageFileName:PA5-87436_N-cadherin_P19022-1_Rabbit.svg,antigenImageFileNamePDP:PA5-87436_N-cadherin_P19022-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.3 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804145

Product Specific Information

Immunogen sequence: NPKIIRQEEG LHAGTMLTTF TAQDPDRYMQ QNIRYTKLSD PANWLKIDPV NGQITTIAVL DRESPNVKNN IYNATFLASD NGIPPMSGTG TLQIYLLDIN DNAPQVLPQE AETCETPDPN SINITALDYD IDPNAGPFAF DLPLSPVTIK RNWTITRLNG DFAQLNLKIK FLEAGIYEVP IIITDSGNPP KSNISILRVK VCQCDSNGDC TDVDRIVGAG LGTGAIIAIL LCIIILLILV LMFVVWMKRR DKERQAKQLL IDPEDDVRDN ILKYDEEGGG EEDQDYDLSQ LQQPDTVEPD A

Positive Samples: Mouse brain, Mouse heart, Rat heart; Cellular Location: Cell membrane, Single-pass type I membrane protein

Target Information

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin`s function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.